Research Paper Volume 13, Issue 16 pp 20016—20028

Oral administration of berberine represses macrophage activation-associated benign prostatic hyperplasia: a pivotal involvement of the NF-κB

Effect of BB on expression patterns of NF-κB p65 and CD68 in testosterone-induced BPH rat model. (A) Fluorescent intensity and co-localization of NF-κB p65 and CD68+ cells in prostate sections from BPH rats. Whole-mount immunohistochemistry of the nucleus (blue), NF-κB p65 (green), and CD68 (red). The overlays show the extent of co-localization (yellow) of NF-κB p65 with macrophage marker CD68. Original magnification 100×.

Figure 5. Effect of BB on expression patterns of NF-κB p65 and CD68 in testosterone-induced BPH rat model. (A) Fluorescent intensity and co-localization of NF-κB p65 and CD68+ cells in prostate sections from BPH rats. Whole-mount immunohistochemistry of the nucleus (blue), NF-κB p65 (green), and CD68 (red). The overlays show the extent of co-localization (yellow) of NF-κB p65 with macrophage marker CD68. Original magnification 100×.