Figure 9. NAC decreased ROS generation in the cardiocytes aging model. H9c2 cells were pre-treated with or without NAC (1 mM), a commonly used ROS scavenger, for 1 hour, and then incubated with or without 10 g/L D-gal for 24 hours. (A) ROS generation was detected using a DCFH-DA probe. Representative fluorescent images showed that NAC decreased ROS generation in the cardiocytes aging model induced by D-gal in vitro. (B) Intracellular ROS was quantified by flow cytometry.