Figure 2. LHX9 overexpression inhibited the growth of glioma cells. (A) Overexpression of Flag-labeled LHX9 in U87 and SK-N-SH cells. Flag-LHX9 expression plasmid was transfected into U87 and SK-N-SH cells using lipofectamine 2000. Cells were screened with puromycin for 1 week, and then Flag-LHX9 expression was identified. (B) The effect of LHX9 expression on the growth of U87 and SK-N-SH cells was detected by CCK8 assay. (C, D) The effect of LHX9 expression on the anchorage-independent growth of U87 and SK-N-SH cells was detected by soft agar assay. (E, F) The EdU assay was performed. Details about the EdU assay were described in the “Materials and methods”. *, P<0.05; **, P<0.01.