Research Paper Volume 13, Issue 18 pp 22109—22119

LHX9, a p53-binding protein, inhibits the progression of glioma by suppressing glycolysis

Interfering with LHX9 expression accelerated the growth of glioma cells. (A) Interference with LHX9 expression in A172 and SK-N-SH cells. The target sequence was cloned into the pL.KO.1 vector, the virus was packaged to infect A172 and SK-N-SH cells. Cells were screened with puromycin for 1 week, and then the expression of LHX9 was identified. (B) The effect of interference with LHX9 expression on the growth of A172 and SK-N-SH cells was detected by CCK8 assay. (C, D) The effect of interference with LHX9 expression on the anchorage-independent growth of A172 and SK-N-SH cells was detected by soft agar assays. *, PP

Figure 3. Interfering with LHX9 expression accelerated the growth of glioma cells. (A) Interference with LHX9 expression in A172 and SK-N-SH cells. The target sequence was cloned into the pL.KO.1 vector, the virus was packaged to infect A172 and SK-N-SH cells. Cells were screened with puromycin for 1 week, and then the expression of LHX9 was identified. (B) The effect of interference with LHX9 expression on the growth of A172 and SK-N-SH cells was detected by CCK8 assay. (C, D) The effect of interference with LHX9 expression on the anchorage-independent growth of A172 and SK-N-SH cells was detected by soft agar assays. *, P<0.05; **, P<0.01.