Research Paper Volume 13, Issue 16 pp 20552—20568

Mediation of circ_RPPH1 on miR-146b-3p/E2F2 pathway to hinder the growth and metastasis of breast carcinoma cells

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Figure 3. circ_RPPH1 acts as miR-146b-3p sponge for regulation. (A) circ_RPPH1 structure. (B) Subcellular localization analysis of circ_RPPH1 distribution in MCF-7 and MDA-MB-231 cells. (C) Circinteractome predicted the target site between circ_RPPH1 and miR-146b-3p. (D) The relative expression of miR-146b-3p in cells transfected with si-circ_RPPH1#2 was detected by qRT-PCR. (E) RIP test detected the targeted binding of circ_RPPH1 and miR-146b-3p. (F) Double luciferase report experimental analysis of targeted binding between circ_RPPH1 and miR-146b-3p. (G) qRT-PCR was used to detect the expression of miR-146b-3p in BC patients. (H) Pearson test was applied to analyze the correlation between miR-146b-3p and circ_RPPH1 in tumor tissues of BC patients. *indicates P < 0.05; **indicates P < 0.01.