Research Paper Volume 13, Issue 16 pp 20552—20568

Mediation of circ_RPPH1 on miR-146b-3p/E2F2 pathway to hinder the growth and metastasis of breast carcinoma cells


Figure 4. miR-146b-3p targets E2F2 and regulates it. (A) Three target genes prediction websites predicted the potential common target gene of miR-146b-3p. (B) GEPIA2 analyzed the relative expression of MED1 and E2F2 in BC tissues in TCGA database. (C) qRT-PCR was used to detect the relative expression of MED1 and E2F2 in BC patients. (D) Pearson test was used to analyze the correlation among circ_RPPH1, miR-146b-3p and E2F2 in tumor tissues of BC patients. (E) Double luciferase report analysis of miR-146b-3p binding to E2F2. (F, G) The relative expression of E2F2 in BC cell lines transfected with miR-146b-3p-mimics and anti-miR-146b-3p was detected by qRT-PCR and WB. *indicates P < 0.05; ***indicates P < 0.001.