Research Paper Volume 13, Issue 17 pp 21232—21250

LncRNA SNHG17 promotes tumor progression and predicts poor survival in human renal cell carcinoma via sponging miR-328-3p

SNHG17 enhanced the expression of H2AX via sponging miR-328-3p. (A) A Venn diagram showed the number of genes identified as potential targets of miR-328-3p according to the three online tools. (B) qRT-PCR assay analysis of miR-328-3p expression in RCC cell lines after transfection of the indicated vectors. (C–D) qRT-PCR and western blot assay analysis of the expression levels of H2AX mRNA (C) and protein (D) in 786-O and ACHN cell lines after transfection with the indicated vectors. (E) The miR-328-3p putative binding sequences and corresponding mutant sites of H2AX 3′UTR. (F) The luciferase activity in RCC cell lines after co-transfection with miR-328-3p mimic or NC mimic and wt or mut constructs of H2AX 3′UTR. (G) western blot assay analyses of the expression levels of H2AX protein in RCC cell lines after transfection with the indicated vectors. (H) Spearman’s correlation analysis of the correlations between expression levels of miR-328-3p (H) or SNHG17 (I) and H2AX in RCC tissues from the Zhengzhou cohort. Data are presented as means ± standard deviation from triplicate experiments. Wt, wild-type; mut, mutant-type; SCR, scramble control; RCC, renal cell carcinoma; ns, not significant. *P **P ***P

Figure 5. SNHG17 enhanced the expression of H2AX via sponging miR-328-3p. (A) A Venn diagram showed the number of genes identified as potential targets of miR-328-3p according to the three online tools. (B) qRT-PCR assay analysis of miR-328-3p expression in RCC cell lines after transfection of the indicated vectors. (CD) qRT-PCR and western blot assay analysis of the expression levels of H2AX mRNA (C) and protein (D) in 786-O and ACHN cell lines after transfection with the indicated vectors. (E) The miR-328-3p putative binding sequences and corresponding mutant sites of H2AX 3′UTR. (F) The luciferase activity in RCC cell lines after co-transfection with miR-328-3p mimic or NC mimic and wt or mut constructs of H2AX 3′UTR. (G) western blot assay analyses of the expression levels of H2AX protein in RCC cell lines after transfection with the indicated vectors. (H) Spearman’s correlation analysis of the correlations between expression levels of miR-328-3p (H) or SNHG17 (I) and H2AX in RCC tissues from the Zhengzhou cohort. Data are presented as means ± standard deviation from triplicate experiments. Wt, wild-type; mut, mutant-type; SCR, scramble control; RCC, renal cell carcinoma; ns, not significant. *P < 0.05; **P < 0.01; ***P < 0.001.