Research Paper Volume 13, Issue 17 pp 21268—21282

Celecoxib reverses the glioblastoma chemo-resistance to temozolomide through mitochondrial metabolism

TMZ and/or celecoxib inhibited colony formation on LN18 and LN229 GBM cells via assay by crystal violet staining. (A) LN18 and LN229 GBM cells (2.5×103) were seeded into 12-well plates, following TMZ and/or celecoxib treatment for 12 days prior to staining. Then the plates were imaged by Bio-Rad equipment. (n=5). (B) After staining of LN18 and LN229 GBM cells, the plates were imaged by EVOS Cell Imaging Systems. (C) The staining plates were calculated by Image Lab software, and the chart was shown the (A) result. Data shows mean Value and ±SEM (n=5).

Figure 3. TMZ and/or celecoxib inhibited colony formation on LN18 and LN229 GBM cells via assay by crystal violet staining. (A) LN18 and LN229 GBM cells (2.5×103) were seeded into 12-well plates, following TMZ and/or celecoxib treatment for 12 days prior to staining. Then the plates were imaged by Bio-Rad equipment. (n=5). (B) After staining of LN18 and LN229 GBM cells, the plates were imaged by EVOS Cell Imaging Systems. (C) The staining plates were calculated by Image Lab software, and the chart was shown the (A) result. Data shows mean Value and ±SEM (n=5).