Research Paper Volume 13, Issue 18 pp 22120—22133

Metformin inhibits hepatocellular carcinoma development by inducing apoptosis and pyroptosis through regulating FOXO3

FOXO3 activates NLRP3 transcription. (A, B) A luciferase activity assay was used to determine whether FOXO3 activated NLRP3 transcription. (C, D) qPCR was used to evaluate the NLRP3 expression after transfection with si-FOXO3 plasmids or vectors overexpressing FOXO3. (E, F) Western blots were used to evaluate NLRP3 expression after transfection with si-FOXO3 plasmids or vectors overexpressing FOXO3. (G) A luciferase activity assay was used to evaluate the transcription activity of NLRP3 after Met treatment. (H) Western blot and (I) qPCR was performed to evaluate the expression of NLRP3 after Met treatment. (J) A ChIP assay detected the interaction between FOXO3 and NLRP3 after Met treatment. *P

Figure 5. FOXO3 activates NLRP3 transcription. (A, B) A luciferase activity assay was used to determine whether FOXO3 activated NLRP3 transcription. (C, D) qPCR was used to evaluate the NLRP3 expression after transfection with si-FOXO3 plasmids or vectors overexpressing FOXO3. (E, F) Western blots were used to evaluate NLRP3 expression after transfection with si-FOXO3 plasmids or vectors overexpressing FOXO3. (G) A luciferase activity assay was used to evaluate the transcription activity of NLRP3 after Met treatment. (H) Western blot and (I) qPCR was performed to evaluate the expression of NLRP3 after Met treatment. (J) A ChIP assay detected the interaction between FOXO3 and NLRP3 after Met treatment. *P<0.05 vs. si-nc or vector or control group.