Figure 3. Overexpressing miR-30b-5p aggravated IL-1β-mediated chondrocyte apoptosis and inflammation. miR-30b-5p mimics and inhibitors were transferred into HC-A cells, respectively. (A) RT-qPCR verified transfection validity. (B, C) CCK8 and flow cytometry tested cell viability and apoptosis, respectively. (D) Profiles of Bax and Cleaved-Caspase3 were examined by WB. (E, F) The expression of IL-1β, TNFα, MMP3, and MMP13 was monitored by RT-qPCR and WB. **P<0.01, ***P<0.001. N=3.