Research Paper Volume 13, Issue 16 pp 20774—20792

NF-κB inducible miR-30b-5p aggravates joint pain and loss of articular cartilage via targeting SIRT1-FoxO3a-mediated NLRP3 inflammasome

miR-30b-5p facilitated NLRP3 expression in chondrocytes and joint tissues. miR-30b-5p mimics or inhibitors were added to HC-A chondrocytes. (A, B) Protein expression of NLRP3-ASC-cleaved Caspase1 was verified by WB after transfecting miR-30b-5p mimics or inhibitors to IL-1β-treated HC-A cells. miR-30b-5p inhibitors were added to the knee joint cavity of OA rats. (C) WB examined the expression of NLRP3-ASC- cleaved Caspase1 in joint tissues after transfecting miR-30b-5p inhibitors. **PP

Figure 5. miR-30b-5p facilitated NLRP3 expression in chondrocytes and joint tissues. miR-30b-5p mimics or inhibitors were added to HC-A chondrocytes. (A, B) Protein expression of NLRP3-ASC-cleaved Caspase1 was verified by WB after transfecting miR-30b-5p mimics or inhibitors to IL-1β-treated HC-A cells. miR-30b-5p inhibitors were added to the knee joint cavity of OA rats. (C) WB examined the expression of NLRP3-ASC- cleaved Caspase1 in joint tissues after transfecting miR-30b-5p inhibitors. **P<0.01, ***P<0.001. N=5.