Research Paper Volume 13, Issue 16 pp 20774—20792

NF-κB inducible miR-30b-5p aggravates joint pain and loss of articular cartilage via targeting SIRT1-FoxO3a-mediated NLRP3 inflammasome

SIRT1 targeted miR-30b-5p. (A, B) The target association between miR-30b-5p and SIRT1 was predicted in the ENCORI database. (C, D) The targeted affinity between miR-30b-5p and SIRT1 was validated by the dual-luciferase reporter assay and RIP experiment, respectively. (E, F) The profiles of SIRT1 and FoxO3a were further evaluated by RT-qPCR and WB. nsP>0.05, ***P

Figure 6. SIRT1 targeted miR-30b-5p. (A, B) The target association between miR-30b-5p and SIRT1 was predicted in the ENCORI database. (C, D) The targeted affinity between miR-30b-5p and SIRT1 was validated by the dual-luciferase reporter assay and RIP experiment, respectively. (E, F) The profiles of SIRT1 and FoxO3a were further evaluated by RT-qPCR and WB. nsP>0.05, ***P<0.001. N=3.