Figure 6. SIRT1 targeted miR-30b-5p. (A, B) The target association between miR-30b-5p and SIRT1 was predicted in the ENCORI database. (C, D) The targeted affinity between miR-30b-5p and SIRT1 was validated by the dual-luciferase reporter assay and RIP experiment, respectively. (E, F) The profiles of SIRT1 and FoxO3a were further evaluated by RT-qPCR and WB. nsP>0.05, ***P<0.001. N=3.