Research Paper Volume 13, Issue 17 pp 21345—21363

Up-regulation of circARF3 reduces blood-brain barrier damage in rat subarachnoid hemorrhage model via miR-31-5p/MyD88/NF-κB axis

The role of circARF3/miR-31-5p axis on OGD/R-mediated microglial inflammation. (A, B) Primary microglia transfected with miR-31-5p mimics and/or circARF3 overexpression plasmids were treated with OGD/R for 4 hours. The levels of IL-1â and TNF-á in the medium supernatant were determined by ELISA. (C) The MyD88-NF-êB pathway activation in microglia was tested by WB. *, **P

Figure 6. The role of circARF3/miR-31-5p axis on OGD/R-mediated microglial inflammation. (A, B) Primary microglia transfected with miR-31-5p mimics and/or circARF3 overexpression plasmids were treated with OGD/R for 4 hours. The levels of IL-1â and TNF-á in the medium supernatant were determined by ELISA. (C) The MyD88-NF-êB pathway activation in microglia was tested by WB. *, **P<0.05, P<0.01vs. OGD/R group. &&, &&& P<0.01, P<0. 001 vs. OGD/R+miR-31-5p group. N=3.