Research Paper Volume 13, Issue 17 pp 21364—21384

Deferoxamine accelerates endothelial progenitor cell senescence and compromises angiogenesis

Deferoxamine-treated early EPCs from young donors mimic the senescent phenotypes of those from old donors. (A) Early EPCs were isolated from young ( 70 year-old) donors for colony formation assays. Early EPCs were incubated with or without deferoxamine DFO (3 μM) in a seven-day culture process. (B–D) Young and old EPCs of the same clone were treated with DFO (3 μM) for four days and then assessed for SA-βGal activity, ATP production and telomere length. * P

Figure 5. Deferoxamine-treated early EPCs from young donors mimic the senescent phenotypes of those from old donors. (A) Early EPCs were isolated from young (< 35 year-old) and senior (> 70 year-old) donors for colony formation assays. Early EPCs were incubated with or without deferoxamine DFO (3 μM) in a seven-day culture process. (BD) Young and old EPCs of the same clone were treated with DFO (3 μM) for four days and then assessed for SA-βGal activity, ATP production and telomere length. * P < 0.05, ** P < 0.01 compared with the untreated young group; # P < 0.05, ## P < 0.01 compared with the untreated old group.