Research Paper Volume 13, Issue 16 pp 20808—20819

LINC01087 inhibits glioma cell proliferation and migration, and increases cell apoptosis via miR-384/Bcl-2 axis

LINC01087 regulates miR-384 to take part in the proliferation and apoptosis of glioma. (A) Fluorescence in situ hybridization determined the distribution of LINC01087 in glioma cells. (B) Targeted binding and mutation sites of miR-384, LINC01087 and Bcl-2. (C) The effect of miR-384-agomiR on fluorescence activity of LINC01087-WT and Bcl-2-WT was tested by dual luciferase report. (D) miR-384 was identified in LINC01087 complex. (E) The relative expression changes of Bcl-2 mRNA and protein in glioma cells after transfection of miR-384-agomiR were tested by qRT-PCR and WB test. (F) The relative expression changes of Bcl-2 mRNA and protein in glioma cells after co-transfection of sh-LINC01087#1 and anti-miR-384 were tested by qRT-PCR and WB test. (G) The correlation between miR-384 and LINC01087, Bcl-2 in glioma patients was analyzed by Pearson test. (*P ***P

Figure 3. LINC01087 regulates miR-384 to take part in the proliferation and apoptosis of glioma. (A) Fluorescence in situ hybridization determined the distribution of LINC01087 in glioma cells. (B) Targeted binding and mutation sites of miR-384, LINC01087 and Bcl-2. (C) The effect of miR-384-agomiR on fluorescence activity of LINC01087-WT and Bcl-2-WT was tested by dual luciferase report. (D) miR-384 was identified in LINC01087 complex. (E) The relative expression changes of Bcl-2 mRNA and protein in glioma cells after transfection of miR-384-agomiR were tested by qRT-PCR and WB test. (F) The relative expression changes of Bcl-2 mRNA and protein in glioma cells after co-transfection of sh-LINC01087#1 and anti-miR-384 were tested by qRT-PCR and WB test. (G) The correlation between miR-384 and LINC01087, Bcl-2 in glioma patients was analyzed by Pearson test. (*P < 0.05, ***P < 0.001).