Figure 1. MiR-185-5p is downregulated in NSCLC tissues and cell lines. The NSCLC tissues and tissues larger than 5 cm around the tumor were collected (N=70, 35 tumor tissues and 35 adjacent tissues). (A) RT-qPCR was used to detect the relative expression of miR-185-5p in tumor tissues and adjacent tissues, and the results showed that miR-185-5p was downregulated in NSCLC tissues. (B) Relative expression of miR-185-5p in NSCLC cell lines (A549, SPC-A1, PC 9, H2170 and SK-MES-1 cells) and normal bronchial epithelial cell line (HBECs) were detected by RT-qPCR assay, and the results showed that miR-185-5p was downregulated in NSCLC cell lines. The miR-185-5p mimic, miR-185-5p inhibitor and their negative control were transfected into A549 cells and H2170 cells, respectively. (C, D) Relative expression of miR-185-5p in A549 cells and H2170 cells was analyzed by RT-qPCR. N=6, * P<0.05, ** P<0.01.