Research Paper Volume 13, Issue 17 pp 21435—21450

MiR-185-5p targets RAB35 gene to regulate tumor cell-derived exosomes-mediated proliferation, migration and invasion of non-small cell lung cancer cells

Exosomes from A549 cells overexpressing miR-185-5p inhibit NSCLC progression in vivo. Sixty-four nude mice were intraperitoneally injected with A549 cells, and then randomly divided into control group (normal saline), EXO group (A549 cell-derived exosomes), NC mimic group (exosomes derived from A549 cells transfected with NC mimic) and miR-185-5p mimic group (exosomes derived from A549 cells transfected with miR-185-5p mimic), with 4 nude mice in each group by intraperitoneal injection. On the days 7, 14, 28 and 35 after injection, three mice were sacrificed in each group, and tumor tissues were taken to determine the tumor volume and weight. (A) Representative tumor images at day 35 post injection. (B) Tumor volume. (C) Tumor weight. We found that injection of exosomes derived from A549 cells transfected with miR-185-5p mimic reduced tumor weight and volume in nude mice. N=4 for each group. **p

Figure 10. Exosomes from A549 cells overexpressing miR-185-5p inhibit NSCLC progression in vivo. Sixty-four nude mice were intraperitoneally injected with A549 cells, and then randomly divided into control group (normal saline), EXO group (A549 cell-derived exosomes), NC mimic group (exosomes derived from A549 cells transfected with NC mimic) and miR-185-5p mimic group (exosomes derived from A549 cells transfected with miR-185-5p mimic), with 4 nude mice in each group by intraperitoneal injection. On the days 7, 14, 28 and 35 after injection, three mice were sacrificed in each group, and tumor tissues were taken to determine the tumor volume and weight. (A) Representative tumor images at day 35 post injection. (B) Tumor volume. (C) Tumor weight. We found that injection of exosomes derived from A549 cells transfected with miR-185-5p mimic reduced tumor weight and volume in nude mice. N=4 for each group. **p< 0.01.