Figure 4. RAB35 is upregulated in NSCLC tissues and cell lines. The NSCLC tissues and tissues larger than 5 cm around the tumor were collected (N=70, 35 tumor tissues and 35 adjacent tissues). (A) RT-qPCR was used to detect the relative expression of RAB35 in tumor tissues and adjacent healthy tissues, and the results showed that RAB35 was upregulated in NSCLC tissues. (B) KM plotter software (http://kmplot.com/analysis/) was used to analyze the expression and survival of RAB35 gene in 1928 lung cancer patients. (C) Western blotting was used to detect the relative protein expression of RAB35 in NSCLC cell lines (A549, SPC-A1, PC 9, H2170 and SK-MES-1 cells) and normal bronchial epithelial cell line (HBECs), and the results showed that RAB35 was upregulated in NSCLC cell lines. (D) RAB35 siRNA and negative control (Scramble) were transfected into A549 cells and H2170 cells, respectively. The protein expression of RAB35 was analyzed by Western botting. β-actin was used as an internal reference. N=6, ** P<0.01.