Research Paper Volume 13, Issue 17 pp 21435—21450

MiR-185-5p targets RAB35 gene to regulate tumor cell-derived exosomes-mediated proliferation, migration and invasion of non-small cell lung cancer cells

MiR-185-5p inhibits proliferation, migration and invasion of NSCLC cells by targeting RAB35. The pcDNA-RAB35 was transfected into A549 cells and H2170 cells alone or together with miR-185-5p mimic, respectively. (A) Fold change of RAB35 protein was analyzed by Western blotting, and we found that miR-185-5p mimic reversed the promoting effect of pcDNA-RAB35 on RAB35 protein expression. (B) The cell proliferation of A549 and H2170 cells at day 1, 2, 3, 4, 5 after transfection was measure by CCK-8 assay. (C) Transwell cell migration assay was used to measure the migration of A549 cells and H2170 cells. (D) The cell invasion was detected by Transwell cell invasion assay. We found that miR-185-5p mimic reversed the promoting effect of pcDNA-RAB35 on cell proliferation, migration and invasion. β-actin was used as an internal reference. N=6, ** P

Figure 6. MiR-185-5p inhibits proliferation, migration and invasion of NSCLC cells by targeting RAB35. The pcDNA-RAB35 was transfected into A549 cells and H2170 cells alone or together with miR-185-5p mimic, respectively. (A) Fold change of RAB35 protein was analyzed by Western blotting, and we found that miR-185-5p mimic reversed the promoting effect of pcDNA-RAB35 on RAB35 protein expression. (B) The cell proliferation of A549 and H2170 cells at day 1, 2, 3, 4, 5 after transfection was measure by CCK-8 assay. (C) Transwell cell migration assay was used to measure the migration of A549 cells and H2170 cells. (D) The cell invasion was detected by Transwell cell invasion assay. We found that miR-185-5p mimic reversed the promoting effect of pcDNA-RAB35 on cell proliferation, migration and invasion. β-actin was used as an internal reference. N=6, ** P<0.01.