Research Paper Volume 13, Issue 17 pp 21435—21450

MiR-185-5p targets RAB35 gene to regulate tumor cell-derived exosomes-mediated proliferation, migration and invasion of non-small cell lung cancer cells

NSCLC cell-derived exosomes with RAB35 knockdown or miR-185-5p overexpression (Enzyme treated) inhibited NSCLC cell migration and invasion. The exosomes secreted by A549 cells and H2170 cells were treated with enzyme and then added into the two kinds of cell models with downregulation of RAB35. (A) Transwell cell migration assay was used to measure the migration of A549 cells and H2170 cells, and the results showed that the promoting effects of exosomes secreted by A549 and H2170 cells on cell migration and invasion were attenuated under the action of enzymes. (B) The cell invasion was detected by Transwell cell invasion assay. N=6, ** P

Figure 9. NSCLC cell-derived exosomes with RAB35 knockdown or miR-185-5p overexpression (Enzyme treated) inhibited NSCLC cell migration and invasion. The exosomes secreted by A549 cells and H2170 cells were treated with enzyme and then added into the two kinds of cell models with downregulation of RAB35. (A) Transwell cell migration assay was used to measure the migration of A549 cells and H2170 cells, and the results showed that the promoting effects of exosomes secreted by A549 and H2170 cells on cell migration and invasion were attenuated under the action of enzymes. (B) The cell invasion was detected by Transwell cell invasion assay. N=6, ** P<0.01.