Research Paper Volume 13, Issue 17 pp 20935—20961

Hyperbaric oxygen therapy alleviates vascular dysfunction and amyloid burden in an Alzheimer’s disease mouse model and in elderly patients

HBOT reduces abnormal processing of APP and attenuates Aβ degradation and clearance in 5XFAD mice. (A) Representative immunoblot assays of the carboxyl-terminal fragment (CTF)β and CTFα. (B, C) Quantification of western blots in (A), presented as percentages of wt control, normalized to GAPDH levels (n = 5–6/group). (D–G) Representative immunoblot assays of IDE protein (D) and LRP1 in (F). (E, G) Quantification of western blots in (D, F), respectively, presented as percentages of wt controls, normalized to GAPDH levels (n = 5-6/group). Two-way ANOVA and post-hoc Fisher LSD tests were performed. Values represent means ± SEM. * P PP P

Figure 3. HBOT reduces abnormal processing of APP and attenuates Aβ degradation and clearance in 5XFAD mice. (A) Representative immunoblot assays of the carboxyl-terminal fragment (CTF)β and CTFα. (B, C) Quantification of western blots in (A), presented as percentages of wt control, normalized to GAPDH levels (n = 5–6/group). (DG) Representative immunoblot assays of IDE protein (D) and LRP1 in (F). (E, G) Quantification of western blots in (D, F), respectively, presented as percentages of wt controls, normalized to GAPDH levels (n = 5-6/group). Two-way ANOVA and post-hoc Fisher LSD tests were performed. Values represent means ± SEM. * P < 0.05, ** P<0.01, *** P < 0.001, **** P < 0.0001.