Research Paper Volume 13, Issue 17 pp 21497—21512

m6A demethylase ALKBH5 suppression contributes to esophageal squamous cell carcinoma progression

RNAi-induced ALKBH5 silencing enhanced the migration and invasion of ESCC cells in vitro. (A, B) Wound healing assays were performed in shALKBH5-expressing ESCC cells. The migration ability was determined by measuring the distance from the boundary of the scratch to the cell-free space after 36 h and 48 h. (C, D) The migration ability of shALKBH5-expressing ESCC cells using the transwell migration assay. The average number of cells per field was calculated from three independent experiments (original magnification: ×200). (E, F) The invasive ability of shALKBH5-expressing ESCC cells using the Boyden invasion assay. The average number of cells per field was calculated from three independent experiments (original magnification: ×200).

Figure 5. RNAi-induced ALKBH5 silencing enhanced the migration and invasion of ESCC cells in vitro. (A, B) Wound healing assays were performed in shALKBH5-expressing ESCC cells. The migration ability was determined by measuring the distance from the boundary of the scratch to the cell-free space after 36 h and 48 h. (C, D) The migration ability of shALKBH5-expressing ESCC cells using the transwell migration assay. The average number of cells per field was calculated from three independent experiments (original magnification: ×200). (E, F) The invasive ability of shALKBH5-expressing ESCC cells using the Boyden invasion assay. The average number of cells per field was calculated from three independent experiments (original magnification: ×200).