Research Paper Volume 13, Issue 17 pp 20962—20991

Glutamine promotes escape from therapy-induced senescence in tumor cells

(A) Dose-dependent effects of glutamine on TIS escape. Doxorubicin-induced senescent MCF-7 and A549 cells were grown in media with different glutamine concentrations. Colonies that evaded the senescent growth arrest were stained and counted. Data are mean ± S.D. of three independent experiments. (B) Dose-dependent effects of glutamine on CD44+/CD24− subpopulation. MCF-7 cells were grown for 48 hours in media with different glutamine concentrations. Expression of CD44 and CD24 was analyzed by flow cytometry. Data are mean ± S.D. of three independent experiments.

Figure 3. (A) Dose-dependent effects of glutamine on TIS escape. Doxorubicin-induced senescent MCF-7 and A549 cells were grown in media with different glutamine concentrations. Colonies that evaded the senescent growth arrest were stained and counted. Data are mean ± S.D. of three independent experiments. (B) Dose-dependent effects of glutamine on CD44+/CD24 subpopulation. MCF-7 cells were grown for 48 hours in media with different glutamine concentrations. Expression of CD44 and CD24 was analyzed by flow cytometry. Data are mean ± S.D. of three independent experiments.