Research Paper Volume 13, Issue 17 pp 21547—21570

HSP90 acts as a senomorphic target in senescent retinal pigmental epithelial cells

IPI-504 inhibit senescent ARPE-19 cells-mediated cell migration. (A) Transwell assay. The chamber with ARPE-19 cells were incubated in wells containing the conditional media from proliferating ARPE-19 cells, day-4 senescent ARPE-19 cells and day 4-senescent ARPE-19 cells that were pretreated with IPI-504 for 24 hours. The migrated cells at bottom were photographed. (B) The quantitation of migrated cell numbers in A. (C) Transwell assay. The chamber with HREC cells were incubated in wells that contains the conditional media used in A. The migrated cells at bottom were photographed. (D) The quantitation of migrated cell numbers in C. The two-tailed unpaired t-test was used for statistical analysis (n = 4).

Figure 7. IPI-504 inhibit senescent ARPE-19 cells-mediated cell migration. (A) Transwell assay. The chamber with ARPE-19 cells were incubated in wells containing the conditional media from proliferating ARPE-19 cells, day-4 senescent ARPE-19 cells and day 4-senescent ARPE-19 cells that were pretreated with IPI-504 for 24 hours. The migrated cells at bottom were photographed. (B) The quantitation of migrated cell numbers in A. (C) Transwell assay. The chamber with HREC cells were incubated in wells that contains the conditional media used in A. The migrated cells at bottom were photographed. (D) The quantitation of migrated cell numbers in C. The two-tailed unpaired t-test was used for statistical analysis (n = 4).