Figure 4. TNFSF9 promotes the migration of pancreatic cancer cells by activating the Wnt/Snail signaling pathway. (A–C) Western blot analysis the expression of Wnt, β-catenin, AKT, p-AKT, JNK, p-JNK, NF-ΚB, p-NF-ΚB, ERK p-ERK, Snail, Src and FAK in BXPC-3 and PANC-1 cells after knockdown of TNFSF9. Compared with the sh-NC group, the expression of Wnt, β-catenin, AKT, p-AKT, JNK, p-JNK, NF-ΚB, p-NF-ΚB and Snail in the sh-TNFSF9-1 and sh-TNFSF9-2 groups is significantly reduced. The expression of ERK, p-ERK, Src and FAK did not change. (D, E) The expression of Wnt, β-catenin, and Snail increased after the addition of Wnt agonist in TNFSF9 knockdown pancreatic cancer, but the expression of TNFSF9 did not change. (F, G) Increased migration of TNFSF9 knockdown pancreatic cancer cells after the addition of Wnt agonist. sh-1 is sh-TNFSF9-1. sh-2 is sh-TNFSF9-2. *P < 0.05, **P < 0.01, ***P < 0.001 and #P < 0.001.