Research Paper Volume 13, Issue 17 pp 21712—21728

The ceRNA PVT1 inhibits proliferation of ccRCC cells by sponging miR-328-3p to elevate FAM193B expression

FAM193B is a miR-328-3p target gene by binding to 3’UTR site. (A) Heat map of down-regulated genes in Caki 1 cells treated with OE-miR-328-3p lentivirus compared with cells treated with lentivirus vector (fold change > 2.0, P B) Venn diagram showing the over-lapping of potential target genes of miR-328-3p in the results of gene chip assay and bioinformatic analysis of PITA, miRmap, and microT. (C) qRT-PCR assays of potential target genes, NOTCH2NL, PDE4B, and FAM193B, in 293T cells treated with lentivirus vector or OE-miR-328-3p lentivirus. (D, E) 786-O and Caki 1 cells treated with lentivirus vector or OE-miR-328-3p lentivirus for 24 hours. FAM193B levels were determined by qRT-PCR. (F) Schematic view of putative miR-328-3p targets in the WT and MUT 3’UTR of FAM193B. (G) Luciferase activity assay of 293 T cells transfected with luciferase reporter plasmids harboring FAM193B 3’UTR (WT or MUT) and control miRNA or miR-328-3p. Mean ± SEM, ** P

Figure 5. FAM193B is a miR-328-3p target gene by binding to 3’UTR site. (A) Heat map of down-regulated genes in Caki 1 cells treated with OE-miR-328-3p lentivirus compared with cells treated with lentivirus vector (fold change > 2.0, P < 0.05). (B) Venn diagram showing the over-lapping of potential target genes of miR-328-3p in the results of gene chip assay and bioinformatic analysis of PITA, miRmap, and microT. (C) qRT-PCR assays of potential target genes, NOTCH2NL, PDE4B, and FAM193B, in 293T cells treated with lentivirus vector or OE-miR-328-3p lentivirus. (D, E) 786-O and Caki 1 cells treated with lentivirus vector or OE-miR-328-3p lentivirus for 24 hours. FAM193B levels were determined by qRT-PCR. (F) Schematic view of putative miR-328-3p targets in the WT and MUT 3’UTR of FAM193B. (G) Luciferase activity assay of 293 T cells transfected with luciferase reporter plasmids harboring FAM193B 3’UTR (WT or MUT) and control miRNA or miR-328-3p. Mean ± SEM, ** P < 0.01, ***P < 0.001, ****P < 0.001, NS: no significance.