Research Paper Volume 13, Issue 17 pp 21712—21728

The ceRNA PVT1 inhibits proliferation of ccRCC cells by sponging miR-328-3p to elevate FAM193B expression

FAM193B is a target of miR-328-3p and is suppressed by PVT1 deletion. (A–C) Correlation between PVT1-miR-328-3p expression levels (A), miR-328-3p-FAM193B expression levels (B) and PVT1-FAM193B expression levels (C) in 45 paired ccRCC tissues. (D) Relative protein levels of FAM193B in DU 145 cells transfected with empty lentivirus vector and OE-miR-328-3p Lentivirus. (E) Relative protein levels of FAM193B in DU 145 cells transfected with empty lentivirus vector and SH-PVT1 Lentivirus. (F) FAM193B mRNA and protein levels in 786-O and Caki-1 cells following knockdown of PVT1 and/or inhibition of miR-328-3p. (G) Protein level in 786-O and Caki-1 cells following knockdown of PVT1 and/or inhibition of miR-328-3p. Mean ± SEM, ** P

Figure 6. FAM193B is a target of miR-328-3p and is suppressed by PVT1 deletion. (AC) Correlation between PVT1-miR-328-3p expression levels (A), miR-328-3p-FAM193B expression levels (B) and PVT1-FAM193B expression levels (C) in 45 paired ccRCC tissues. (D) Relative protein levels of FAM193B in DU 145 cells transfected with empty lentivirus vector and OE-miR-328-3p Lentivirus. (E) Relative protein levels of FAM193B in DU 145 cells transfected with empty lentivirus vector and SH-PVT1 Lentivirus. (F) FAM193B mRNA and protein levels in 786-O and Caki-1 cells following knockdown of PVT1 and/or inhibition of miR-328-3p. (G) Protein level in 786-O and Caki-1 cells following knockdown of PVT1 and/or inhibition of miR-328-3p. Mean ± SEM, ** P < 0.01, ****P < 0.001, NS: no significance.