Research Paper Volume 13, Issue 17 pp 21729—21742

Cytokine antibody array-based analysis of IL-37 treatment effects in asthma

Immunohistochemistry detection of IL-18Ra, IL-18Rb, SIGIRR in mice lung tissue from PBS, OVA/PBS, OVA/rhIL-37 group (n=10 mice per group). Representative photomicrographs of immunohistochemistry staining for IL-18Ra (A), IL-18Rb (B) and SIGIRR (C) in mice lung tissue from each group. (D) Quantitation of IL-18Ra, IL-18Rb, SIGIRR expression. IL-18Ra and SIGIRR expression in the lung tissue of the OVA/IL-37 group was significantly upregulated compared to the OVA/PBS group, while the difference of IL-18Rb expression in the lung tissue of the OVA/IL-37 group was not statistically significant upregulated compared to the OVA/PBS group. Columns and error bars represented mean±SEM. n=10 per group. **p

Figure 4. Immunohistochemistry detection of IL-18Ra, IL-18Rb, SIGIRR in mice lung tissue from PBS, OVA/PBS, OVA/rhIL-37 group (n=10 mice per group). Representative photomicrographs of immunohistochemistry staining for IL-18Ra (A), IL-18Rb (B) and SIGIRR (C) in mice lung tissue from each group. (D) Quantitation of IL-18Ra, IL-18Rb, SIGIRR expression. IL-18Ra and SIGIRR expression in the lung tissue of the OVA/IL-37 group was significantly upregulated compared to the OVA/PBS group, while the difference of IL-18Rb expression in the lung tissue of the OVA/IL-37 group was not statistically significant upregulated compared to the OVA/PBS group. Columns and error bars represented mean±SEM. n=10 per group. **p< 0.01, ***p<0.001. ****p<0.0001.