Figure 2. Low HCG11 expression in OS is a result of transcriptional repression of YY1. (A) HCG11 expression in OS cells detected by qRT-PCR after YY1 overexpression or knockdown. (B) Potential YY1 recognition sites in the promoter region of HCG11. (C) The combination of YY1 to the promoter region of HCG11 was validated by ChIP assay. (D) Several predicted binding sites were verified by luciferase assays. (E) YY1 mRNA expression in OS tissues and ANTs was analyzed by qRT-PCR. (F) Correlation between YY1 expression and HCG11 expression was analyzed by Spearman’s analysis. NS, no significance, *P < 0.05, **P < 0.01, ***P < 0.001.