Research Paper Volume 13, Issue 17 pp 21040—21065

Knock-down of odr-3 and ife-2 additively extends lifespan and healthspan in C. elegans

Kaplan-Meier survival curves depicting the effects of combined genetic interventions on odr-3, ife-2 and cku-70 at 20° C. (A–D) Lifespan comparisons in the WT background (continuous lines). (E–H) Lifespan comparisons in the daf-16(mu86) background (dashed lines). Survival curves represent: (A, E) odr-3(n1605) and ife-2(RNAi) single and double genetic interventions; (B, F) odr-3(n1605) and cku-70(RNAi) single and double genetic interventions; (C, G) ife-2(RNAi) and cku-70(RNAi) single and double genetic interventions; (D, H) odr-3(n1605), ife-2(RNAi) and cku-70(RNAi) double and triple genetic interventions. (C, D) Control in the case of single RNAi knock-downs refers to treatment with a 1:1 mixture of RNAi bacteria and EV bacteria, in order to be comparable to the double RNAi intervention. (A–H) The survival plots in the WT background represent pooled populations from 3 independent experiments, whereas survival plots in the daf-16(m28) background represent pooled populations from 2 independent experiments. odr-3 denotes odr-3(n1605) fed with EV; daf-16 denotes daf-16(mu86) fed with EV; all strains in these experiments were grown on agar plates with E. coli HT115(DE3) and FUdR.

Figure 1. Kaplan-Meier survival curves depicting the effects of combined genetic interventions on odr-3, ife-2 and cku-70 at 20° C. (AD) Lifespan comparisons in the WT background (continuous lines). (EH) Lifespan comparisons in the daf-16(mu86) background (dashed lines). Survival curves represent: (A, E) odr-3(n1605) and ife-2(RNAi) single and double genetic interventions; (B, F) odr-3(n1605) and cku-70(RNAi) single and double genetic interventions; (C, G) ife-2(RNAi) and cku-70(RNAi) single and double genetic interventions; (D, H) odr-3(n1605), ife-2(RNAi) and cku-70(RNAi) double and triple genetic interventions. (C, D) Control in the case of single RNAi knock-downs refers to treatment with a 1:1 mixture of RNAi bacteria and EV bacteria, in order to be comparable to the double RNAi intervention. (AH) The survival plots in the WT background represent pooled populations from 3 independent experiments, whereas survival plots in the daf-16(m28) background represent pooled populations from 2 independent experiments. odr-3 denotes odr-3(n1605) fed with EV; daf-16 denotes daf-16(mu86) fed with EV; all strains in these experiments were grown on agar plates with E. coli HT115(DE3) and FUdR.