Research Paper Volume 13, Issue 17 pp 21040—21065

Knock-down of odr-3 and ife-2 additively extends lifespan and healthspan in C. elegans

Kaplan-Meier survival curves for animals containing the odr-3(n1605) and ife-2(ok306) mutations. (A) odr-3(n1605); ife-2(ok306) single and double mutants, cultivated in the presence of FUdR. (B) odr-3(n1605); ife-2(ok306) single and double mutants, cultured without FUdR. (C) Lifespan comparisons for odr-3 and ife-2 in the daf-16(mu86) background. (A–C) Dashed lines are used for odr-3(n1605) and ife-2(ok306) mutants tested in the daf16(mu86) genetic background, while continuous lines are used for WT or single/double odr-3 and ife-2 mutants tested in the WT background. All cohorts were fed OP50 and kept at 20° C. All lifespan values can be viewed in the Supplementary Table 1.

Figure 3. Kaplan-Meier survival curves for animals containing the odr-3(n1605) and ife-2(ok306) mutations. (A) odr-3(n1605); ife-2(ok306) single and double mutants, cultivated in the presence of FUdR. (B) odr-3(n1605); ife-2(ok306) single and double mutants, cultured without FUdR. (C) Lifespan comparisons for odr-3 and ife-2 in the daf-16(mu86) background. (AC) Dashed lines are used for odr-3(n1605) and ife-2(ok306) mutants tested in the daf16(mu86) genetic background, while continuous lines are used for WT or single/double odr-3 and ife-2 mutants tested in the WT background. All cohorts were fed OP50 and kept at 20° C. All lifespan values can be viewed in the Supplementary Table 1.