Research Paper Volume 13, Issue 18 pp 22208—22231

Involvement of c-Myc in low dose radiation-induced senescence enhanced migration and invasion of unirradiated cancer cells

LDR induced cell senescence but not apoptosis. (A and B) MTT assay for analyzing the cell viability of LDR treated WI-38 cells and H1299 cells, respectively. (C and D) Western blot analysis of Caspase-3 and Caspase-9 for detecting the cleaved proteins in LDR treated WI-38 cells and H1299 cells, respectively. (E) SA-β-gal analysis for determining the level of cell senescence induced by LDR. (F) Quantification of X-gal stained cells before and after LDR. *p

Figure 2. LDR induced cell senescence but not apoptosis. (A and B) MTT assay for analyzing the cell viability of LDR treated WI-38 cells and H1299 cells, respectively. (C and D) Western blot analysis of Caspase-3 and Caspase-9 for detecting the cleaved proteins in LDR treated WI-38 cells and H1299 cells, respectively. (E) SA-β-gal analysis for determining the level of cell senescence induced by LDR. (F) Quantification of X-gal stained cells before and after LDR. *p < 0.05.