Research Paper Volume 13, Issue 18 pp 22242—22255

miR-21 regulates ischemic neuronal injury via the p53/Bcl-2/Bax signaling pathway

MiR-21 regulated the p53/Bcl-2/Bax signaling following ischemia in vivo. (A) qRT-PCR indicated the effect of miR-21 mimic and mimic control on the expression of miR-21 following I/R in vivo. (B, C) qRT-PCR (B) and western blot (C) indicated the effect of miR-21 mimic and mimic control on mRNA and protein levels of p53, Bax, and Bcl-2 following I/R in vivo. (D) qRT-PCR indicated the effect of miR-21 inhibitor and NC inhibitor on the expression of miR-21 following I/R in vivo. (E, F) qRT-PCR (E) and western blot (F) indicated the effect of miR-21 inhibitor and NC inhibitor on mRNA and protein levels of p53, Bax, and Bcl-2 following I/R in vivo. Eight mice were randomly selected from each treatment group. *, compared with the sham group. *P≤0.05, **P≤0.01, ***P≤0.001. #, mimic control + MCAO group vs. miR-21 mimic + MCAO group, or NC inhibitor + MCAO group vs. miR-21 inhibitor + MCAO group. #P≤0.05, ##P≤0.01.

Figure 5. MiR-21 regulated the p53/Bcl-2/Bax signaling following ischemia in vivo. (A) qRT-PCR indicated the effect of miR-21 mimic and mimic control on the expression of miR-21 following I/R in vivo. (B, C) qRT-PCR (B) and western blot (C) indicated the effect of miR-21 mimic and mimic control on mRNA and protein levels of p53, Bax, and Bcl-2 following I/R in vivo. (D) qRT-PCR indicated the effect of miR-21 inhibitor and NC inhibitor on the expression of miR-21 following I/R in vivo. (E, F) qRT-PCR (E) and western blot (F) indicated the effect of miR-21 inhibitor and NC inhibitor on mRNA and protein levels of p53, Bax, and Bcl-2 following I/R in vivo. Eight mice were randomly selected from each treatment group. *, compared with the sham group. *P≤0.05, **P≤0.01, ***P≤0.001. #, mimic control + MCAO group vs. miR-21 mimic + MCAO group, or NC inhibitor + MCAO group vs. miR-21 inhibitor + MCAO group. #P≤0.05, ##P≤0.01.