Research Paper Volume 13, Issue 18 pp 22276—22285

Improvement of cardiac function by mesenchymal stem cells derived extracellular vesicles through targeting miR-497/Smad7 axis

Direct binding site between Smad7 and miR-497 was identified. (A) The binding site between Smad7 and miR-497 was predicted; (B) The binding site between Smad7 and miR-497 was verified; (C) Influence of EVs and miR-497 mimics on the expression of Smad7 was measured using IHC staining; (D) Influence of EVs and miR-497 mimics on the expression of Smad7 was analyzed; (E) Influence of EVs and miR-497 mimics on the expression of Smad7 was measured using western blotting; (F) Influence of EVs and miR-497 mimics on the protein expression of Smad7 was analyzed; (G) Influence of EVs and miR-497 mimics on the mRNA expression of Smad7 was analyzed. + indicated P

Figure 4. Direct binding site between Smad7 and miR-497 was identified. (A) The binding site between Smad7 and miR-497 was predicted; (B) The binding site between Smad7 and miR-497 was verified; (C) Influence of EVs and miR-497 mimics on the expression of Smad7 was measured using IHC staining; (D) Influence of EVs and miR-497 mimics on the expression of Smad7 was analyzed; (E) Influence of EVs and miR-497 mimics on the expression of Smad7 was measured using western blotting; (F) Influence of EVs and miR-497 mimics on the protein expression of Smad7 was analyzed; (G) Influence of EVs and miR-497 mimics on the mRNA expression of Smad7 was analyzed. + indicated P<0.05 compared with the group sham. * indicated P<0.05 compared with the group MI. # indicated P<0.05 compared with group MI+EVs.