Research Paper Volume 13, Issue 18 pp 22276—22285

Improvement of cardiac function by mesenchymal stem cells derived extracellular vesicles through targeting miR-497/Smad7 axis

Knockdown of Smad7 reversed the improvement of cardiac function induced by EVs. (A) Influence of EVs and sh-Smad7 on the histological changes of MI animals; (B) Collagen deposition was investigated using Masson trichrome staining; (C) Collagen deposition was investigated using sirius red staining; (D) Influence of EVs and sh-Smad7 on infarction was analyzed; (E) Influence of EVs and sh-Smad7 on left ventricular fractional shortening was analyzed; (F) Influence of EVs and sh-Smad7 on left ventricular ejection fraction was analyzed; (G) Influence of EVs and sh-Smad7 on MDA, SOD, CAT, and GSH-Px was analyzed; (H) sh-Smad7 reversed the influence of EVs on the expression of miR497. + indicated P

Figure 5. Knockdown of Smad7 reversed the improvement of cardiac function induced by EVs. (A) Influence of EVs and sh-Smad7 on the histological changes of MI animals; (B) Collagen deposition was investigated using Masson trichrome staining; (C) Collagen deposition was investigated using sirius red staining; (D) Influence of EVs and sh-Smad7 on infarction was analyzed; (E) Influence of EVs and sh-Smad7 on left ventricular fractional shortening was analyzed; (F) Influence of EVs and sh-Smad7 on left ventricular ejection fraction was analyzed; (G) Influence of EVs and sh-Smad7 on MDA, SOD, CAT, and GSH-Px was analyzed; (H) sh-Smad7 reversed the influence of EVs on the expression of miR497. + indicated P<0.05 compared with the group sham. * indicated P<0.05 compared with the group MI. # indicated P<0.05 compared with group MI+EVs.