Research Paper Volume 13, Issue 18 pp 22432—22443

Circular RNA Foxo3 enhances progression of ovarian carcinoma cells

Foxo3 is transported through exosome-mediated intercellular communication. (A) The morphology of exosomes, as determined by TEM. Scale bar = 100 μm. (B) The size distribution of exosomes, as determined by NTA. (C) The protein expressions of exosomal markers Tsg101 and CD63 in exosomes isolated from SKOV3 and control cells. (D) The expression of Foxo3 in exosomes derived from the control or SKOV3 cells. (E) The expression of Foxo3 in exosomes with Foxo3 knockdown or overexpressing. (F) Visualization of exosomes taken up by SKOV3 cells, as observed by fluorescence microscope. Scale bar = 25 μm. (G) The expression of Foxo3 in SKOV3 cells co-cultured with exosomes with Foxo3 knockdown or overexpressing. (H) Cell proliferation of SKOV3 cells co-cultured with exosomes with Foxo3 knockdown or overexpressing, as determined by CCK-8 assay. Data were expressed as means ± standard deviation. For each treatment, three replicates were used. **p ***p

Figure 5. Foxo3 is transported through exosome-mediated intercellular communication. (A) The morphology of exosomes, as determined by TEM. Scale bar = 100 μm. (B) The size distribution of exosomes, as determined by NTA. (C) The protein expressions of exosomal markers Tsg101 and CD63 in exosomes isolated from SKOV3 and control cells. (D) The expression of Foxo3 in exosomes derived from the control or SKOV3 cells. (E) The expression of Foxo3 in exosomes with Foxo3 knockdown or overexpressing. (F) Visualization of exosomes taken up by SKOV3 cells, as observed by fluorescence microscope. Scale bar = 25 μm. (G) The expression of Foxo3 in SKOV3 cells co-cultured with exosomes with Foxo3 knockdown or overexpressing. (H) Cell proliferation of SKOV3 cells co-cultured with exosomes with Foxo3 knockdown or overexpressing, as determined by CCK-8 assay. Data were expressed as means ± standard deviation. For each treatment, three replicates were used. **p < 0.01, ***p < 0.001.