Figure 7. Norbixin inhibits the transactivation of RXRs induced by A2E. Effect of increasing concentrations of A2E on RXR transactivation (A). Effect of A2E (20 μM) and HX630 (5 μM) on RXR transactivation (B). Effect of A2E (20 μM), NBX (20 μM) and A2E (20 μM) + NBX (20 μM) on RXR transactivation (C). Effect of HX630 (5 μM) alone or in competition with NBX (20 μM) on RXR transactivation (D). Bars represent mean ± s.e.m. with n = 3–5. ** or ##p < 0.01, ***p < 0.001, ****p < 0.0001 compared to CONT (DMSO alone) or to A2E, respectively (One-way ANOVA, Dunnett's post-test).