COVID-19 Priority Research Paper Volume 13, Issue 18 pp 21838—21854

SARS-CoV-2 causes senescence in human cells and exacerbates the senescence-associated secretory phenotype through TLR-3

Toll-like receptor-3 (TLR-3) is increased in senescent vs. non-senescent human kidney endothelial cells and preadipocytes. (A) TLR expression (rtPCR) in radiation-induced senescent vs. non-senescent human preadipocytes (n=3). (B) TLR (rtPCR) in radiation-induced senescent human kidney endothelial cells (n=4) vs. non-senescent cells. Data are expressed as a function of non-senescent cells; mean +/- SEM, paired (A), unpaired (B), 2-tailed Student’s t-tests. (C) TLR-3 protein (Western blots) in non-senescent and senescent preadipocytes (n=3) and kidney endothelial cells (n=3) with tubulin as loading control (optical density of TLR-3 as a function of α-tubulin [%]). Data are expressed as a function of non-senescent cells; mean +/- SEM, paired (preadipocytes), unpaired (kidney endothelial cells), 2-tailed Student’s t-tests.

Figure 1. Toll-like receptor-3 (TLR-3) is increased in senescent vs. non-senescent human kidney endothelial cells and preadipocytes. (A) TLR expression (rtPCR) in radiation-induced senescent vs. non-senescent human preadipocytes (n=3). (B) TLR (rtPCR) in radiation-induced senescent human kidney endothelial cells (n=4) vs. non-senescent cells. Data are expressed as a function of non-senescent cells; mean +/- SEM, paired (A), unpaired (B), 2-tailed Student’s t-tests. (C) TLR-3 protein (Western blots) in non-senescent and senescent preadipocytes (n=3) and kidney endothelial cells (n=3) with tubulin as loading control (optical density of TLR-3 as a function of α-tubulin [%]). Data are expressed as a function of non-senescent cells; mean +/- SEM, paired (preadipocytes), unpaired (kidney endothelial cells), 2-tailed Student’s t-tests.