Research Paper Volume 13, Issue 18 pp 22556—22570

MSCs enhances the protective effects of valsartan on attenuating the doxorubicin-induced myocardial injury via AngII/NOX/ROS/MAPK signaling pathway

MSCs combined with Val effects on ROS generation, AngII, NOX2, NOX4, caspase-3 and cleaved caspase-9 expression in H9c2 cells. (A) Schematic diagram of co-culture of MSCs and H9c2 cells. (B) Western blot analysis of MSCs and Val-treatment on AT1R, NOX2, NOX4, caspase-3, cleaved caspase-9 proteins levels. (C) Effect of MSCs and Val-treatment on ROS levels at 12 h time point in H9c2 cells. (D, E) Densitometry analysis of the protein bands of AT1R, NOX2, NOX4, caspase-3, cleaved caspase-9 proteins. (F) Microscopic analysis of MSCs and Val-treatment on ROS levels by DCF Fluorescence. *P

Figure 5. MSCs combined with Val effects on ROS generation, AngII, NOX2, NOX4, caspase-3 and cleaved caspase-9 expression in H9c2 cells. (A) Schematic diagram of co-culture of MSCs and H9c2 cells. (B) Western blot analysis of MSCs and Val-treatment on AT1R, NOX2, NOX4, caspase-3, cleaved caspase-9 proteins levels. (C) Effect of MSCs and Val-treatment on ROS levels at 12 h time point in H9c2 cells. (D, E) Densitometry analysis of the protein bands of AT1R, NOX2, NOX4, caspase-3, cleaved caspase-9 proteins. (F) Microscopic analysis of MSCs and Val-treatment on ROS levels by DCF Fluorescence. *P<0.05 vs. Control; †P<0.05 vs. Dox; #P<0.05 vs. Dox+Val, Dox+MSCs. Dox, doxorubicin; Val, valsartan; MSCs, mesenchymal stem cells.