Research Paper Volume 13, Issue 22 pp 24560—24579

Circular RNA CELF1 drives immunosuppression and anti-PD1 therapy resistance in non-small cell lung cancer via the miR-491-5p/EGFR axis

Forced expression of circ

Figure 2. Forced expression of circ_CELF1 induces the progression of NSCLC cells in vitro and in vivo. (A) The qRT-PCR was performed to confirm the transfection efficiency of circ_CELF1 in NSCLC cells. n = 5, *P < 0.05. (B) CCK-8 assay was performed to detect the cell viability on A549 and H1975 cells. n = 6, *P < 0.05. (C) The cell cycle was analyzed on A549 and H1975 cells. n = 3, *P < 0.05. (D) The clone formation assay on A549 and H1975 cells. n = 3, *P < 0.05. (E) The wound healing assay was performed on A549 and H1975 cells. n = 3, *P < 0.05. (F) The ability of invasion was explored on A549 and H1975 cells by Transwell. n = 4, *P < 0.05. (G) Tumor growth in NSCLC cells with the forced expression of circ_CELF1 was investigated by xenograft tumor models. n = 7, *P < 0.05. (H) The number of metastatic nodules of the lung was counted in two groups. n = 6, *P < 0.05.