Research Paper Volume 13, Issue 22 pp 24560—24579

Circular RNA CELF1 drives immunosuppression and anti-PD1 therapy resistance in non-small cell lung cancer via the miR-491-5p/EGFR axis

Forced expression of miR-491-5p remits the cell progression in stabled circ

Figure 4. Forced expression of miR-491-5p remits the cell progression in stabled circ_CELF1 expression NSCLC cells. (A) CCK-8 assay was performed to detect the effect of miR-491-5p on overexpression of circ_CELF1 A549 and H1975 cells. n = 6, *P < 0.05. (B) The cell cycle was explored by flow cytometry on overexpression of circ_CELF1 A549 and H1975 cells after miR-491-5p transfection. n = 6, *P < 0.05. (C) The clone formation assay was performed on overexpression of circ_CELF1 A549 and H1975 cells. n = 5, *P < 0.05. (D) Wound healing assay was used to confirm the migration ability. n = 5, *P < 0.05. (E) The migration and invasion ability was explored by Transwell. n = 6, *P < 0.05, **P < 0.01.