Research Paper Volume 13, Issue 22 pp 24560—24579

Circular RNA CELF1 drives immunosuppression and anti-PD1 therapy resistance in non-small cell lung cancer via the miR-491-5p/EGFR axis

EGFR is a target of miR-491-5p. (A) Three bioinformatics sites predict the target of miR-491-5p. (B) The prediction binding sites between EGFR and miR-491-5p (upper). Luciferase assay confirmed the relationship between EGFR and miR-491-5p (lower). n = 3, *P C and D) The expression of EGFR was detected by qRT-PCR in A549 and H1975 cells after circ

Figure 5. EGFR is a target of miR-491-5p. (A) Three bioinformatics sites predict the target of miR-491-5p. (B) The prediction binding sites between EGFR and miR-491-5p (upper). Luciferase assay confirmed the relationship between EGFR and miR-491-5p (lower). n = 3, *P < 0.05. (C and D) The expression of EGFR was detected by qRT-PCR in A549 and H1975 cells after circ_CELF1/miR-491-5p transfection. n = 3, *P < 0.05. (E) H&E staining and EGFR staining in tumor tissue and matched adjacent normal tissues. (F) The expression of EGFR in 37 cases of NSCLC tissues was detected by qRT-PCR. A positive correlation between circ_CELF1 and EGFR was observed in tumor tissues at the mRNA levels. n = 37, *P < 0.05. (G) A negative correlation between miR-491-5p and EGFR was observed in tumor tissues at the mRNA levels. n = 37, *P < 0.05.