Figure 2. ZnO NPs repress global protein synthesis. (A) Measurement of the newly synthesized proteins with different treatments using puromycin labeling. Cells were treated with 5 μg/mL of ZnO NPs. (B) Polysome profiles of A549 cells treated without or with ZnO NPs (5 μg/mL) or together with NAC. The ratio of polysome/monosome was calculated. (C) Immunoblot analysis of A549 cells without or with ZnO NPs (5 μg/mL) treatment or together with NAC.