Research Paper Volume 13, Issue 19 pp 23119—23132

Ribosome profiling reveals translatome remodeling in cancer cells in response to zinc oxide nanoparticles

ZnO NPs repress global protein synthesis. (A) Measurement of the newly synthesized proteins with different treatments using puromycin labeling. Cells were treated with 5 μg/mL of ZnO NPs. (B) Polysome profiles of A549 cells treated without or with ZnO NPs (5 μg/mL) or together with NAC. The ratio of polysome/monosome was calculated. (C) Immunoblot analysis of A549 cells without or with ZnO NPs (5 μg/mL) treatment or together with NAC.

Figure 2. ZnO NPs repress global protein synthesis. (A) Measurement of the newly synthesized proteins with different treatments using puromycin labeling. Cells were treated with 5 μg/mL of ZnO NPs. (B) Polysome profiles of A549 cells treated without or with ZnO NPs (5 μg/mL) or together with NAC. The ratio of polysome/monosome was calculated. (C) Immunoblot analysis of A549 cells without or with ZnO NPs (5 μg/mL) treatment or together with NAC.