Research Paper Volume 13, Issue 21 pp 24071—24085

LncRNA MIR31HG is induced by tocilizumab and ameliorates rheumatoid arthritis fibroblast-like synoviocyte-mediated inflammation via miR-214-PTEN-AKT signaling pathway

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Figure 3.

The ceRNA network for MIR31HG-miR-214-PTEN in RA-FLS. (A) The endogenous expression of miR-214 in OA and RA synovium samples (n=6). (B) The endogenous expression of miR-214 in RA-FLS following tocilizumab treatment (n=4). (C) The effects of MIR31HG siRNAs on miR-214 expression in RA-FLS (n=3). (D) Bioinformatic prediction of binding between MIR31HG and miR-214. (E) The expression of MIR31HG in miR-214 overexpressing RA-FLS (n=3). (F) Luciferase reporter assay demonstrating the direct interactions between miR-214 and MIR31HG (n=3). (G) Bioinformatic prediction of binding between the PTEN 3’ UTR and miR-214. (H) The expression of PTEN in miR-214 overexpressing RA-FLS (n=3). (I) Luciferase reporter assay demonstrating the direct interactions between miR-214 and the PTEN 3′ UTR (n=3). (J) Western blot showing the endogenous expression of PTEN in MIR31HG knockdown RA-FLS (n=3). Data represent the mean ± SEM; *p < 0.05, as determined by the Student’s t-test.