Effects of sinomenine on CCI rats. (A) The behavioral mechanical allodynia and thermal hyperalgesia of CCI rats treated with sinomenine at days 14 after operation. (B) The mRNA expressions of TNF-α, IL-1β and IL-6 in the lumbar spinal cords of rats treated with sinomenine via qRT-PCR at 14 days after operation. (C) The levels of RIP3, p-JNK, JNK, c-Fos, and GAPDH in the lumbar spinal cords of rats treated with sinomenine at 14 days after operation. (D) The ratio of RIP3, c-Fos to GAPDH and the ratio of p-JNK to JNK were analyzed. (E) The expression of RIP3 in lumbar spinal cord of rats treated with sinomenine by immunofluorescence on 14th day after operation. (F) Quantification of immunofluorescence area of (C) with Image J. Data are shown as mean SD (n = 8). *P < 0.05, contrasted to the CCI group.