Research Paper Volume 13, Issue 22 pp 24640—24654

PPARα agonist relieves spinal cord injury in rats by activating Nrf2/HO-1 via the Raf-1/MEK/ERK pathway

PPARα agonist promoted motor function repair in SCI rats by activating the Raf-1/MEK/ERK signaling pathway. (A–G) The rats were randomly divided into sham-operation group (Sham group), rat SCI model group (SCI group), SCI + PPARα agonist PEA group (PEA group) and SCI + PEA + Raf-1 inhibitor GW5074 group (GW group). The rats in the PEA group were intraperitoneally injected with PEA (2 mg/kg). The GW group were administered with Raf-1 inhibitor GW5074 (5 mg/kg) based on the PEA group treatment. The expression of the V-raf-1 murine leukemia viral oncogene homolog 1 (Raf-1)/mitogen-activated protein kinase (MEK)/extracellular signal-regulated kinase (ERK) signaling pathway-related protein detected by Western Blotting in spinal cord tissues; The BBB score; HE staining (scale bar = 50 μm); The number of neurons; The expression of ROS (ACR and MnSOD) detected by IHC in spinal cord tissues; The expression of apoptosis factors (Bax and Bcl-2) detected by Western Blotting in spinal cord tissues; Protein expression levels of nuclear factor erythroid 2-related factor 2 (Nrf2), heme oxygenase 1 (HO-1) and thioredoxin-1 (Trx-1) detected by Western Blotting in spinal cord tissues; (H) A diagram model of this study. ‘*’ indicates p

Figure 6. PPARα agonist promoted motor function repair in SCI rats by activating the Raf-1/MEK/ERK signaling pathway. (AG) The rats were randomly divided into sham-operation group (Sham group), rat SCI model group (SCI group), SCI + PPARα agonist PEA group (PEA group) and SCI + PEA + Raf-1 inhibitor GW5074 group (GW group). The rats in the PEA group were intraperitoneally injected with PEA (2 mg/kg). The GW group were administered with Raf-1 inhibitor GW5074 (5 mg/kg) based on the PEA group treatment. The expression of the V-raf-1 murine leukemia viral oncogene homolog 1 (Raf-1)/mitogen-activated protein kinase (MEK)/extracellular signal-regulated kinase (ERK) signaling pathway-related protein detected by Western Blotting in spinal cord tissues; The BBB score; HE staining (scale bar = 50 μm); The number of neurons; The expression of ROS (ACR and MnSOD) detected by IHC in spinal cord tissues; The expression of apoptosis factors (Bax and Bcl-2) detected by Western Blotting in spinal cord tissues; Protein expression levels of nuclear factor erythroid 2-related factor 2 (Nrf2), heme oxygenase 1 (HO-1) and thioredoxin-1 (Trx-1) detected by Western Blotting in spinal cord tissues; (H) A diagram model of this study. ‘*’ indicates p < 0.05.