Research Paper

Figure 3. SOX4 was a direct target of miR-34a. (A) Multiple databases (Targetscan, miRanda and TarBase) identified that the SOX4 had complementary base pairs with miR-34a. (B) WT and mutant SOX4 luciferase plasmids were transfected into HEK293 cells with miR-34a mimics or NC. The luciferase activity was measured by dual-luciferase reporter assay system. (^*p < 0.05). miR-34a or miR-34a inhibitor or its NC was transfected into TCP-1 cells. (C) The mRNA level of SOX4 was analyzed by qRT-PCR, (D) and the protein level of SOX4 was tested by Western blot (^*p < 0.05). The protein expression of SOX4 in PTC tissues (E) and cells (F) was detected by Western blot (^*p < 0.05). (G) The correlation analysis of miR-34a and SOX4 expression in PTC tissues.