Figure 5.FOXN3-AS2 directly regulates miR-34a and inhibits its expression in PTC. (A) Starbase, LncBook and DIANA databases used to identify the lncRNAs with miR-34a biding sites. (B) WT and mutant miR-34a luciferase plasmids were transfected into HEK293 cells with pcDNA3.1 or FOXN3-AS2. The luciferase activity was measured by dual-luciferase reporter assay system (*p < 0.05). (C) FOXN3-AS2 or si-FOXN3-AS2 or its NC was transfected into TCP-1 cells. The mRNA level of FOXN3-AS2, miR-34a and SOX4 were analyzed by qRT-PCR (*p < 0.05). (D) FOXN3-AS2 and miR-34a were cotransfected into TCP-1 cells, the mRNA level of SOX4 were tested (*p < 0.05). The protein level of miR-34a in PTC tissues (E) and cells (F) was detected by qRT-PCR (*p < 0.05). (G) The correlation analysis of miR-34a and FOXN3-AS2 expression in PTC tissues.
