Figure 1. Expression of Fam114A1 in the normal and vitiligo melanocytes. (A) Comparing the growth rate of melanocytes from three different sources, it can be seen that the growth rate of melanocytes from around the skin lesions or normal parts of patients with vitiligo and the immortalized cell line PI3V of vitiligo are significantly slower than normal melanocytes. Statistical analyses were performed using one-way ANOVA and Dunnett’s post hoc test:*P<0.05, **P < 0.01 MC vs MCV-Lor MCV-N or PI3V. (B) The expression of Fam114A1 in melanocytes from different sources was detected by immunofluorescence (200×). (C) Immunohistochemical analysis of vitiligo skin lesions and normal human skins showed that Fam114A1 protein was highly expressed in the local skin lesions of vitiligo; (D, E) The expression of Fam114A1 in vitiligo melanocytes (MCV-L, MCV-N, PI3V) was confirmed by western blot, and significantly statistical results was shown. Statistical analyses were performed vs. MC using one-way ANOVA and Dunnett’s post hoc test: **P < 0.01 vs. MC.