Research Paper Volume 13, Issue 22 pp 24740—24752

Figure 4. The role of RACK1 in Fam114A1 regulating MC apoptosis. (A, B) Expression of RACK1 protein in MC from different sources, the expression of RACK1 protein in vitiligo-derived MC is lower than that of normal MC. Statistical analyses were performed using one-way ANOVA and Dunnett’s post hoc test: **P < 0.01 vs MC; ***P<0.001 vs MC. (C, D) The apoptosis rate of Fam114A1-RACK1+ was decreased compared with Fam114A1+RACK1+, while the apoptosis rate of Fam114A1-RACK1- was increased compared with both Fam114A1+RACK1+ and Fam114A1-RACK1+. Statistical analyses were performed using one-way ANOVA and Dunnett’s post hoc test: **P < 0.01; ***P<0.001. (E, F) The expression of apoptosis-related proteins in Fam114A1+RACK1+, Fam114A1-RACK1+,RACK1and Fam114A1-RACK1- were detected by western blot, it can be seen that the expression of apoptotic proteins bax, caspase3, cleaved-caspase3, and P53 decreased significantly in Fam114A1-RACK1+ compared with Fam114A1+RACK1+ and increased significantly in Fam114A1-RACK1- compared with both Fam114A1+RACK1+ and Fam114A1-RACK1+, the result of bcl2 is just the opposite. Statistical analyses were performed using one-way ANOVA and Dunnett’s post hoc test: *P < 0.05, **P < 0.01, ***P<0.001.